The invention provides a lung targeting cefquinome sulfate EC microsphere and a preparation method thereof and belongs to the technical field of antibiotic targeting preparation for animals. The preparation method comprises the following steps: uniformly mixing and ball-milling bulk drugs with ethyl alcohol, isopropanol and dichloromethane and drying liquid, thereby acquiring the treated bulk drugs; taking ethyl cellulose and albumin as a carrier, dissolving in an ethanol solution, heating, stirring and waiting for dissolving, and then adding cefquinome, ultrasonically circulating and fully and uniformly mixing; adding flow agent and suspending agent and uniformly mixing; performing spray drying, and meanwhile, magnetically stirring and then acquiring the microsphere through an eddy separator. The encapsulation efficiency of the microsphere provided by the invention is above 90%, the drug loading capacity is above 15% and more than 90% of microsphere grain size is distributed within 10-25um; the operation is simple and convenient, the efficiency is high, the condition is mild and the grain size is conveniently controlled; the dispersity is better and the appearance is uniform; the microsphere has better slow release effect and targeting property and is safe and reliable; the detection method is excellent in specificity, high in sensitivity and high in recovery rate.