The invention discloses an Au complexed Fe3O4 graft block copolymer hybrid material with multiple responses and a preparation method of the Au complexed Fe3O4 graft block copolymer hybrid material. Firstly, aminated Fe3O4 and 2-bromoisobutyryl bromide are subjected to a reaction, an atom transfer radical polymerization initiator is introduced to the Fe3O4 surface, then the Fe3O4 surface is modified with hydroxyethyl acrylate and a tert-butyl methacrylate monomer with an atom transfer radical polymerization method, after hydrolysis as well as esterification with mercaptosuccinic acid and a magnetic block copolymer, a product is complexed with Au nanosol, and the Au complexed Fe3O4 graft block copolymer hybrid material with pH response, electrochemical response and magnetic response is obtained. Spherical micelles are formed by the hybrid material in an aqueous solution, the particle size distribution is in the range of 85-155 nm, the hybrid material has high Zeta potential and high stability, pH transition point is 5.3-5.9, and the hybrid material can make rapid response to different acid-base environments; electrochemical response and plasma resonance of the hybrid material are improved effectively by means of compounding of Au nanoparticles.