The invention relates to a preparation method of a pure coenzyme Q10 product. The method comprises the following steps: carrying out ceramic membrane microfiltration and spray drying on a coenzyme Q10 fermentation liquid to obtain a crude product, extracting the crude product with acetone, carrying out phase separation and reduced pressure concentration on the obtained extract liquid to obtain a coenzyme Q10 extract concentrate, extracting the coenzyme Q10 extract concentrate with petroleum ether, carrying out silica gel column chromatography, carrying out reduced pressure distillation concentration on the above obtained eluate, adding coenzyme Q10 crystal seeds to crystallize the obtained eluate concentrate, and carrying out reduced pressure drying to obtain the pure coenzyme Q10. Mycelia are crushed through ceramic membrane microfiltration and spray drying, acetone refluxing extraction and ceramic membrane filter separation are carried out to realize wall breaking and separation of mycelium cells, and the coenzyme Q10 is enriched, so the method increases the extraction yield by 7% or more, and facilitates the increase of the economic benefit; the method is simple, and is suitable for industrial production; and the method reduces the wastewater discharge amount, and facilitates environmental protection treatment.