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发明公开
CN107338225A 猪支气管上皮细胞系、制备方法及其应用 有权

猪支气管上皮细胞系、制备方法及其应用
摘要：
本发明提供猪支气管上皮细胞系、制备方法及其应用，属于生物技术领域。猪支气管上皮细胞系hTERT-PBEC，保藏编号为CCTCC NO：C201749。该细胞系hTERT-PBEC的制备方法，包括构建携带绿色荧光蛋白基因和人端粒酶逆转录酶基因的真核表达载体pEGFP-hTERT；利用Lipofectamin 3000将真核表达载体pEGFP-hTERT转染猪原代支气管上皮细胞，通过G418筛选得到猪支气管上皮细胞系hTERT-PBEC。本发明通过巧妙的方法，显著提高了筛选效率，得到的猪支气管上皮细胞系，能够稳定传代至60代以上，且保留有原代细胞的特性，能够应用于研究猪呼吸道病原感染致病机理中。
摘要（英）：

The invention provides a porcine bronchial epithelial cell line and a preparation method and application thereof, and belongs to the field of biological technology. The porcine bronchial epithelial cell line is hTERT-PBEC, and the preservation number is CCTCCNO:C201749. The preparation method of the cell line hTERT-PBEC includes constructing of eukaryotic expression vector pEGFP-hTERT carrying green fluorescent protein gene and human telomerase reverse transcriptase gene; using of Lipofectamin3000 for transfection of primary porcine bronchial epithelial cells with the eukaryotic expression vector pEGFP-hTERT, and G418 screening to obtain the porcine bronchial epithelial cell line hTERT-PBEC. The screening efficiency is remarkably improved through ingenious method, and the obtained porcine bronchial epithelial cell line can steadily pass over 60 generations, retains the characteristics of the primary cells, and can be applied to study on the pathogenic mechanism of porcine respiratory tract pathogen infection.

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