Tropilaelaps spp. are important pest mites of Apidae in the Asian region. The invention discloses application of Chinese honeylocust abnormal fruits in control of Tropilaelaps spp. of bees, and provides Chinese honeylocust abnormal fruit powder and Chinese honeylocust abnormal fruit compound powder and a preparation method thereof. The Chinese honeylocust abnormal fruits used as a mite killing component are plant tissue, which are more environmentally friendly and pollution-free for bee products and safe for adult bees, young bees, ova and queen bees; by means of ultra-fine pulverization, the granularity of the Chinese honeylocust abnormal fruit powder and the Chinese honeylocust abnormal fruit compound powder is made finer, the utilization rate of effective components is higher, and the effect of control of Tropilaelaps spp. of the bees is better than that of sublimed sulfur.