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CN107354131A 一种用于脐带间充质干细胞的培养基 无效 - 驳回 转让

一种用于脐带间充质干细胞的培养基
摘要：
本发明公开了一种用于脐带间充质干细胞的培养基，由以下组分组成：基础培养基、人血清白蛋白、杨梅素、三七总皂苷、大黄素、亚油酸、胰蛋白酶、抑肽酶、光蛋白聚糖、碱性成纤维细胞生长因子、叶酸、β-巯基乙醇、黄芪多糖、转铁蛋白、维生素、氨基酸。本发明的培养基将基础培养基、人血清白蛋白、杨梅素、三七总皂苷、大黄素、亚油酸、胰蛋白酶、抑肽酶、光蛋白聚糖、碱性成纤维细胞生长因子、叶酸、β-巯基乙醇、黄芪多糖、转铁蛋白、维生素、氨基酸进行复配，各组分起到协同作用，能够成功培养脐带间充质干细胞，促进脐带间充质干细胞增殖，提高脐带间充质干细胞的生长速率，提高脐带间充质干细胞在体外增殖分化的活性，降低细胞凋亡率。
摘要（英）：

The invention discloses a medium for umbilical cord MSCs (mesenchymal stem cells). The medium is prepared from the following components: a basal medium, human serum albumin, myricetin, panax notoginsenoside, emodin, linoleic acid, trypsin, aprotinin, lumican, basic fibroblast growth factors, folic acid, beta-mercaptoethanol, astragalus polysaccharides, transferrin, vitamins and amino acid. The medium is compounded from the basal medium, human serum albumin, myricetin, panax notoginsenoside, emodin, linoleic acid, trypsin, aprotinin, photoprotective glycoprotein, the basic fibroblast growth factors, folic acid, beta-mercaptoethanol, astragalus polysaccharides, transferrin, the vitamins and the amino acid, the components play a synergistic role, and accordingly, the umbilical cord MSCs can be successfully cultured, proliferation of the umbilical cord MSCs is promoted, the growth rate of the umbilical cord MSCs is increased, activity of the umbilical cord MSCs during in-vitro proliferation and differentiation is improved, and the apoptosis rate is decreased.

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