The invention discloses a one-pot preparation method for an electrochemiluminescence material. The method includes the steps that a mixture solution of cadmium chloride, thiol hydrochloride and sodium hydrogen telluride is prepared, wherein the molar ratio of cadmium chloride to thiol hydrochloride to sodium hydrogen telluride is 1: (1-3.0): (0.04-0.1); the mixture solution is subjected to heating reflux for 0.5-10.5 hours at 115-125 DEG C under a stirring condition, and then the electrochemiluminescence material is obtained. Thiol hydrochloride is hydrochloride of a compound shown in the chemical formula 1, and the electrochemiluminescence material is cadmium telluride quantum dots coated by a compound shown in the chemical formula 1. The invention further discloses the electrochemiluminescence material prepared by the adoption of the one-pot preparation method of the material. The one-pot method for preparing the electrochemiluminescence material is simple, efficient, convenient, low in cost and wide in application range, the purpose of large-scale application is easy to achieve, and industrial production is easy to achieve; the prepared electrochemiluminescence material can also generate strong luminous signals under the condition that a solution does not contain a co-reaction agent, and not only is the situation avoided that the co-reaction agent is introduced into the solution, but also the efficiency and signals of electrochemiluminescence can be effectively improved.