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发明授权
CN107356687B 一种丙氨酰-酪氨酸含量的检测方法 有权

一种丙氨酰-酪氨酸含量的检测方法
摘要：
本发明涉及检测技术领域，特别涉及一种丙氨酰‑酪氨酸含量的检测方法。该检测方法包括：取供试品溶液和丙氨酰‑酪氨酸对照品溶液采用高效液相色谱技术进行检测，根据外标法得到供试品溶液中丙氨酰‑酪氨酸的含量；高效液相色谱技术的色谱柱为C18色谱柱，等度洗脱，流动相由流动相A和流动相B组成，流动相A为水，流动相B为乙腈，流动相A在流动相中的体积百分含量≥80％，流动相B在流动相中的体积百分含量≤20％。本发明提供的检测方法可准确检测样品中丙氨酰‑酪氨酸的含量；且空白、检出限、定量限、线性、精密度、稳定性、回收率试验均符合要求，证明含量测定方法科学有效。
摘要（英）：

The invention relates to the technical field of detection, and especially relates to a detection method of the content of alanyl-tyrosine. The detection method comprises the following steps: taking a sample solution and an alanyl-tyrosine reference substance solution, detecting the solutions through high performance liquid chromatography, and carrying out an external standard method to obtain the content of alanyl-tyrosine in the sample solution, wherein the chromatographic column of the high performance liquid chromatography is a C18 chromatographic column, isocratic elution is carried out, a mobile phase comprises, by volume, 80% or more of a mobile phase A and 20% or less of a mobile phase B, the mobile phase A is water, and the mobile phase B is acetonitrile. The detection method can accurately detect the content of the alanyl-tyrosine in the sample; and blank, detection limit, quantitation limit, linearity, precision, stability and recovery rate tests meet requirements, so it is proved that the content detection method is scientific and effective.

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