The invention discloses a manufacturing method of an organic light-emitting diode. The manufacturing method comprises the steps that an anode layer is formed on a substrate; a doped material layer is formed on the anode layer; the doped material layer is immersed in a poly(3,4-ethylenedioxy thiophene)-poly(styrene sulfonic acid) solution so that the poly(3,4-ethylenedioxy thiophene)-poly(styrene sulfonic acid) solution is enabled to permeate into the doped material layer to be mixed with the doped material layer so as to form a mixture layer; the mixture layer is dried so as to form a hole injection layer; and a light-emitting layer, an electron transmission layer, an electron injection layer and a cathode layer are formed on the hole injection layer in turn. The invention also discloses the organic light-emitting diode. With application of the method, the light emission efficiency and the light emission uniformity of the organic light-emitting diode can be enhanced.