The invention discloses a liquid-phase stripping method of a two-dimensional molybdenum disulfide (MoS2) nano material, and a dispersion method and application of MoS2. The molybdenum disulfide dispersant comprises a polyaniline conductive high polymer which enables molybdenum disulfide to be stably dispersed in a conventional solvent or any other dispersion medium by being combined with molybdenum disulfide under physical actions. The low-cost polyaniline conductive high polymer is used as the molybdenum disulfide dispersant, and the dispersant is simply mixed with the molybdenum disulfide in the dispersion medium, so that a simple liquid-phase stripping method can be utilized to obtain molybdenum disulfide two-dimensional nanosheets under the physical interactions between the dispersing agent and molybdenum disulfide. The liquid-phase stripping method does not damage the physical structure or chemical properties of the molybdenum disulfide, and is simple in the operational process and beneficial to large-scale implementation. The stripped molybdenum disulfide two-dimensional nanosheets have wide application prospects in the fields of semiconductors, energy sources, wear-resistant lubricating paints, composite materials and the like.