The invention discloses a glass composition, and a preparation method and application thereof. The glass composition comprises 66-72 mol% of SiO2, 11-13 mol% of Al2O3, 1.5-3 mol% of MgO and CaO, 14-20 mol% of Na2O, 0.5-1 mol% of K2O and 0.6-2 mol% of V2O5, summing to 100 mol%, wherein the content of MgO is no less than 1 .5 mol%. The invention also provides the preparation method for the glass composition and application of the glass composition to preparation of a touch screen. The glass composition disclosed in the invention has the advantages of short strengthening time and good strengthening effect; after strengthening for 2 h, the glass composition has strengthening depth of up to 50 [mu]m; after strengthening, the glass composition has compressive stress of no less than 972 MPa and Vickers hardness of no less than 644 Kgf/mm<2>; and the glass composition improves anti-falling capability of glass and is not prone to devitrification.