The invention discloses a cryopreservation method of rhynchostylis retusa pollen. The cryopreservation method comprises the following steps: collecting and drying the pollen, freezing and thawing. In the pollen drying process, the pollen is placed in a breathable bag, a drying agent is isolated from the pollen to prevent pollen pollution, the breathable bag is good in breathability, and the drying effect is very good; furthermore, the concentration of DMSO in a vitrifying solution PVS2 is reduced, propylene glycol is added on the basis of original components of the PVS2, and the propylene glycol is an anti-freezing agent with permeability and low toxicity. Therefore, by the cryopreservation method of the rhynchostylis retusa pollen, provided by the invention, the pollen vitality can be effectively guaranteed for a long time; the cryopreservation method is simple and easy to implement; the pollen can be taken out and thawed for pollination at any time, so that the cryopreservation method has an important practicing and promotional significance in breeding and production of rhynchostylis retusa.