The invention discloses a soil sterilization regeneration successive cropping obstacle resistance composition as well as a preparation method and application thereof. The composition is prepared from special microorganisms, organic catalysts, mineral, surfactants and organic biomass, and achieves the effects of creating the soil strong reductive environment in an accelerated way in anaerobic environment, killing soil-borne pathogenic bacteria in a short period, improving the soil microbial community structures, improving the soil pH and eliminating secondary salinization, so that the soil is restored so as to control the successive cropping obstacle; the mu yield can be improved; the effects in aspects of soil restoration, soil successive cropping obstacle control and the like are obvious.