The invention discloses a novel chiral aggregation-induced emission material and a preparation method and application thereof. The structural formula of the chiral aggregation-induced emission material is as shown in formula (II). The preparation method comprises the following steps: firstly designing and synthesizing a type of novel tetraphenyl ethylene phosphate, wherein the structural formula of the novel tetraphenyl ethylene phosphate is as shown in formula (I), and enabling the tetraphenyl ethylene phosphate to react with (R)-[1,1'-binaphthyl]-2,2'-biformaldehyde so as to generate the novel aggregation-induced emission material with the chiral structure. The chiral aggregation-induced emission material has the good optical property. In a mixed system of tetrahydrofuran and water, an ultraviolet-visible absorption spectrum and a fluorescence emission spectrum of the chiral aggregation-induced emission material are changed along with the change of optical rotation. The material can be stimulated by 451 nm of blue light in the solid state, and emits 520 nm of green light. The solid-state fluorescence quantum yield is 29%. The chiral aggregation-induced emission material can be used as the chiral material applied to the fields, such as a photoelectric device and a biosensor, and has the larger application prospect.