Disclosed is a method for establishing a software test case generation technology. The method includes the steps of 1), collecting a source code of to-be-tested software; 2), setting a fitness function; 3), defining an aging factor and setting an aging factor threshold value; 4), establishing a reborn differential evolution method and a result output program and setting up an application system; 5), setting up a test case generation platform in combination with the application system and a test tool; 6), initializing population and generating initial population randomly; 7), acquiring a coverage rate of a test case generated randomly; 8), completing rebirth evolution of the population; 9), outputting a test case suite that meets the requirements. By the above steps, establishment of the software test case generation technology can be completed on the basis of the reborn differential evolution method; the method is applicable to solving the problem about test case design in actual software test, a software tester can be assisted in reducing cost and time, and the test case suite high in flaw detectability is designed.