The invention discloses a production technology of selenium-rich Mingliu highland barley wine and relates to the technical field of wine production. The production technology of the selenium-rich Mingliu highland barley wine comprises the steps as follows: highland barley is crushed, washed and screened, then, raw materials are added to the highland barley for digestion, fermentation is performed, and the product is obtained after filtration. According to the production technology of selenium-rich Mingliu highland barley wine, the highland barley wine contains microelement selenium due to addition of selenium-rich water, so that microelements essential to the human body are supplemented, the nutrition of the human body is guaranteed, the effects of repairing cardiac muscle, removing free radicals in the body, delaying aging and regulating the microcirculatory system of the human body are realized, the Keshan disease and the Kaschin-Beck disease are avoided, the stability of the human body and functions is effectively maintained, so that the highland barley wine has a healthcare function and makes the body of a drinker healthier.