The invention discloses an anti-shake spraying box. The anti-shake spraying box comprises a box body; the box body is internally provided with an anti-shake mechanism; the anti-shake mechanism comprises a supporting rod fixedly arranged at the inner bottom of the box body and also comprises a circular suspension plate; the supporting rod passes through the center of the circular suspension plate to ensure that the supporting rod and the circular suspension plate are coaxially arranged and the circular suspension plate can move on the supporting rod along the length direction of the supporting rod; a plurality of through holes are formed in the surface of the circular suspension plate; the upper end of the supporting rod is provided with a first stopping part, and the lower end of the supporting rod is provided with a second stopping part; and the circular suspension plate is located between the first stopping part and the second stopping part. The problem that the flight stability of an unmanned aerial vehicle is affected by the side-to-side strenuous shake of a pesticide in the box body in the flight process due to a relatively simple structure of a pesticide spraying box on the common unmanned aerial vehicle is solved.