The invention discloses a preparation method of a double-gene time sequence sustained-release tissue engineering scaffold material. The preparation method comprises the following steps: firstly, encapsulating plasmid containing IGF-I by virtue of a sustained-release material which is relatively high in molecular weight, so that sustained-release microspheres are prepared; and preparing the three-dimensional porous tissue engineering material from the microspheres, PLGA which is relatively low in molecular weight and plasmid containing BMP-2 by virtue of a supercritical CO2 and particulate leaching method. As the material is implanted into a body, a main body material, which is relatively low in molecular weight, around the BMP-2 plasmid which is not encapsulated in sustained-release microspheres is degraded firstly, and the BMP-2 plasmid is released firstly; while the IGF-I plasmid, which is encapsulated in the material, which is relatively high in molecular weight, is delayed in release, so that an effect that two target genes are released in a certain order. According to the material, cell factors can be subjected to time sequence expression in a mode of simulating a physiological process of tissue repair, so that the material is more conducive to tissue regeneration and systemic side effects are reduced; a gene product can achieve local continuous release; and a local therapeutic effect can be enhanced to the greatest extent.