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发明公开
CN107418953A 一种从大刍草里扩增玉米磷信号响应基因的方法 无效 - 驳回

一种从大刍草里扩增玉米磷信号响应基因的方法
摘要：
本发明公开了一种大刍草里扩增玉米磷信号响应基因的方法。该方法包括在大刍草里提取待测玉米磷信号响应基因总DNA，分别利用5个引物对组成的引物组进行PCR扩增反应，分别进行克隆测序，最后拼接序列，得到玉米磷信号响应基因全基因组序列。5个引物对的核苷酸序列如SEQ ID NO.4-SEQ ID NO.13所示。本发明还公开了一种上述的引物组和方法在玉米磷信号响应基因或基因组扩增方面的应用。本发明首次利用PCR扩增技术在大刍草里扩增出玉米磷信号响应基因的基因序列。本发明的引物对扩增出来的目的带亮且单一，证明本发明的引物对的特异性好。
摘要（英）：

The invention discloses a method for amplifying corn phosphorus signal response genes from teosinte. The method includes the steps: extracting total DNA (deoxyribonucleic acid) of corn phosphorus signal response genes from the teosinte; performing PCR (polymerase chain reaction) by a primer group comprising five primer pairs; performing cloning and sequencing; finally, splicing sequences to obtain a whole genome sequence of the corn phosphorus signal response genes. Nucleotide sequences of the five primer pairs are as shown in SEQ ID NO. 4 to SEQ ID NO. 13. The invention further discloses an application of the primer group and the method to the corn phosphorus signal response genes or genome amplification. The PCR technology is used for amplifying gene sequences of the corn phosphorus signal response genes from the teosinte for the first time. A target band amplified by the primer pairs is bright and single, which proves that the primer pairs are good in specificity.

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