The invention relates to a permanent magnet repulsion type magnetic suspension linear guide rail and belongs to the technical field of transmission engineering components. The permanent magnet repulsion type magnetic suspension linear guide rail comprises a guide rail, guide rail magnetic strips, a sliding block body, an adjusting block, a lateral sliding body magnetic stripe, an upper sliding block body magnetic stripe, guide pin shafts, an adjusting screw, a locking screw, side plates and side plate screws. According to the permanent magnet repulsion type magnetic suspension linear guide rail, the four pairs of permanent magnet repulsion type magnetic suspension magnet set magnetic strips are correspondingly mounted on the guide rail and the sliding block body, the repulsion acting directions of two pairs of magnetic suspension magnet sets are the up-down directions, the repulsion acting directions of the two pairs of magnetic suspension magnet sets are the left-right internally-inclined directions, and the stable repulsion magnetic suspension structure is formed; and gaps between the magnetic strips of the two pairs of magnetic suspension magnet sets on the two inclined side faces can be adjusted. According to the permanent magnet repulsion type magnetic suspension linear guide rail, the guide rail is in non-mechanical contact with the sliding block body by the adoption of the permanent magnet repulsion magnetic suspension, and the guide rail has the beneficial effects of being free of mechanical wear and free of lubricating and can be applied to a precision machine. The guide rail has the beneficial effects of being good in repulsion suspension stability, high in antijamming capability, adjustable in bearing rigidity, good in applicability, compact and simple in structure and easy to apply and popularize.