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发明公开
CN107421938A 一种检测孔雀石绿的SiO2@ROX纳米粒子荧光探针阵列的制备方法 有权

一种检测孔雀石绿的SiO2@ROX纳米粒子荧光探针阵列的制备方法
摘要：
一种检测孔雀石绿的SiO2@ROX纳米粒子荧光探针阵列的制备方法，包括用APTS与有机染料ROX进行偶联，在碱性条件下与TEOS水解合成表面功能化修饰氨丙基的SiO2@ROX纳米粒子，在刻蚀的硅片上制备出SiO2@ROX纳米粒子荧光探针阵列。本发明的制备过程包括如下三个步骤：首先是在乙醇体系中制备ROX-APTS前驱体，然后，运用溶胶-凝胶法将氨丙基和ROX染料功能化修饰到SiO2纳米粒子表面，最后，将SiO2@ROX纳米粒子荧光探针滴加到在刻蚀微洞的硅片上，抹去表面多余的粒子，制备出比表面积大、识别位点多、敏感性强的SiO2@ROX纳米粒子荧光探针阵列选择性识别和痕量检测目标分析物孔雀石绿。
摘要（英）：

The invention discloses a method for preparing a SiO2@ROX nanoparticle fluorescent probe array for detecting malachite green. The method comprises the following steps: coupling APTS (3-Amimopropyl Triethoxysilane) and an organic dye ROX (5-Carboxy-X-Rhodamine N-Succinimidyl Ester), and dehydrating with TEOS (Tetraethylorthosilicate) under an alkali condition to synthesize SiO2@ROX nanoparticles of surface functionalized modified aminopropyl; and preparing the SiO2@ROX nanoparticle fluorescent probe array on an etched silicon wafer. The method comprises the following three steps: firstly, preparing an ROX-APTS precursor in an ethanol system, performing functionalized modification on aminopropyl and an ROX dye onto the surfaces of SiO2 nanoparticles by using a sol-gel method, and finally dropping a SiO2@ROX nanoparticle fluorescent probe onto a silicon wafer etched with micro-pores, and removing excessive particles on the surface, thereby obtaining the SiO2@ROX nanoparticle fluorescent probe array which is large in specific surface area, rich in recognition site and good in sensitivity for selective recognition and trace detection on a target analyte, namely malachite green.

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