The invention discloses a road and bridge height limitation frame. The road and bridge height limitation frame comprises two stand columns, bases are arranged at the lower ends of the two stand columns, the height limitation frame can be conveniently moved by arranging pulleys under the bases, and when the height limitation frame is used, the height of a beam is adjusted to limited height. A height limitation warning board displays the meter of height limitation and is set to be an LED lamp board, and therefore a driver still can clearly see the numbers on the height limitation warning board even in darkness; an infrared height measurement instrument is installed on an upper beam, when a vehicle exceeding the limited height is driven to the height limitation frame within a certain distance range, the infrared height measurement instrument receives signals, meanwhile, a safety warning lamp on the upper beam flicks, a warning loudspeaker gives a prompt to the driver, and at the moment, an electric telescopic vehicle-stopping rod descends to stop the vehicle exceeding the limited height. The height limitation frame has effective prompting and alarming functions, and effectively stops the vehicle exceeding the limited height, and traffic monitoring is made easier through a camera on the upper beam.