The invention discloses a method for detecting temperature tolerance of gel microspheres, and belongs to the field of oil exploitation. The method comprises the following steps: acquiring an aqueous solution with preset mass concentration of the gel microspheres; correspondingly acquiring unit volume absorbancy of the aqueous solution of the gel microspheres before and after being heated; acquiring particle retention rate of the gel microspheres according to the unit volume absorbancy; determining the temperature tolerance of the gel microspheres according to the particle retention rate. According to the method, the unit volume absorbancy of the aqueous solution of the gel microspheres before and after being heated is obtained, and accordingly the particle retention rate of the gel microspheres can be obtained, thus the quantity of the gel microspheres molten to form liquid due to the temperature influence can be determined, and as a result, the temperature tolerance of the gel microspheres can be quantitatively determined. With the adoption of the method, manual error can be avoided, and the temperature tolerance of the gel microspheres can be accurately and quickly determined.