The invention provides a liquid incoming valve which is mainly used for a chromatographic column and comprises a nozzle, a valve seat, a hollow cylinder, a liquid incoming pipe and a cleaning pipe. A glue spraying pipe and the nozzle are pulled to move downwards or upwards together through the cylinder to realize opening and closing of the valve so as to complete filling of chromatographic filler or cleaning of the chromatographic column and the valve itself. The liquid incoming valve is simple and reliable in structure and compact and small in size, labor intensity of working personnel is lowered greatly, and working efficiency is improved.