The invention relates to a cigarette bobbin paper additive for increasing sour and sweet note of cigarette and a preparation method and application of the cigarette bobbin paper additive. The cigarette bobbin paper additive is prepared by mixing 0.02% to 0.06% of 2-methyl isoamyl butyrate, 0.21% to 0.26% of isoamyl isovalerate, 0.05% to 0.09% of anisic aldehyde and 99.72% to 99.59% of alcohol or propylene glycol in volume percent, oscillating for 10 to 20min under a room temperature to prepare a homogenous solution, putting at a shade place away from light, and storing for 24 to 48 hours to obtain the cigarette bobbin paper additive. The additive is sprayed to the surface of cigarette bobbin paper according to 2.0 to 3.0mL/hectogram of bobbin paper; thus, the aroma volume of the cigarette is increased, obvious sour and sweet note is given to the cigarette, fragrance is rich, stimulation is reduced, and cigarette smoking satisfaction and enjoyment are improved; meanwhile, the cigarette bobbin paper additive further can improve smelt aroma of the cigarette and improve comfortableness of sidestream smoke.