The invention discloses an application method of a packer rubber cylinder for oil well mining. Firstly, a multilayered structure is arranged on a cylinder body, axial load can be borne to generate radial deformation, so that the outer wall of the rubber cylinder and the wall of a casing pipe generate contact pressure, annulus is packed, the rubber cylinder is placed between an oil pipe and the casing pipe in the working process, metal tube heads at the two ends of the cylinder body are connected with the casing pipe, and an inner adhesive layer mainly achieves the buffer function; the mutual action between the cylinder body and the wall of the casing pipe in the sealing process is reduced, a self-sealing unit arranged on an outer adhesive layer can generate deformation when the cylinder body bears radial stress, and after the deformation quantity of each of a trapezoidal block and a parallelogram block reaches to a maximum value, internal stress can be generated in the axial direction to contend with external force; and the trapezoidal block accords with the parallelogram block, internal molecule of the outer adhesive layer is prevented from conducting relative sliding due to the action of the internal stress, and a formed groove has a certain adjusting function on the mutual action of the trapezoidal block and the parallelogram block.