The invention provides an apolipoprotein A1 detection kit. The apolipoprotein A1 detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises 0.8 to 51g/L of octylphenoxypolyethoxyethanol; the reagent R2 comprises 0.8 to 51g/L of octylphenoxypolyethoxyethanol, 0.01 to 15.5g/L magnesium salt, 0.01 to 20g/L of lime acetate, and an antibody. The kit is good in antibody performance and repeatability, and accurate in reagent detection result, and can meet the use requirement.