The invention discloses a laser printing film and a preparation method thereof and belongs to the technical field of laser printing materials. The laser printing film comprises a base material. One side of the base material is provided with an antistatic layer, and the other side of the base material is provided with a powdered ink receiving layer. The antistatic layer comprises an antistatic layer adhesive and an antistatic agent, the antistatic agent is an ethoxy nonionic fluorocarbon surfactant, and the molecular structure of the ethoxy nonionic fluorocarbon surfactant contains an element (the formula can be found in specification), wherein n is larger than or equal to 2. The laser printing film is excellent in appearance quality, stable in antistatic effect, high in image resolution ratio and stable in printing quality, and the antistatic effect maintains 10<5>-10<11> omega/cm<2>.