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发明公开
CN107475268A 来源于木霉的脂肪酶基因及其相关产品与应用 失效 - 权利终止

来源于木霉的脂肪酶基因及其相关产品与应用
摘要：
本发明公开了来源于木霉的脂肪酶基因及其相关产品与应用。该脂肪酶基因是编码序列为序列2的第13-1659位的TlLipA基因或编码序列为序列2的第1-1659位的RTlLipA基因。TlLipA基因或RTlLipA基因表达的蛋白质具有脂肪酶活性，该蛋白质的脂肪酶的最适pH是9.5、最适温度是50℃，该蛋白质的脂肪酶活性可被表面活性剂Triton X100、Tween 20和SDS增强；该蛋白质在pH值6-9的条件下脂肪酶活性稳定。TlLipA基因或RTlLipA基因表达的蛋白质是对表面活性剂具有强耐受性的碱性嗜温脂肪酶，可应用于洗涤行业。
摘要（英）：

The invention discloses lipase gene derived from trichoderma, and relative products and application thereof. The lipase gene adopts TlLipA gene with the coded sequence of 13rd to 1659th of a sequence 2 or RTlLipA gene with the coded sequence of 1st to 1659th of the sequence 2. Protein expressed by the TlLipA gene or the RTlLipA gene has lipase activity, the optimum pH of lipase of the protein is 9.5, the optimum temperature is 50 DEG C, and the lipase activity of the protein can be enhanced by surfactants Triton X100, Tween 20 and SDS; the lipase activity of the protein is stable in the condition that the pH value is 6 to 9. The protein expressed by the TlLipA gene or the RTlLipA gene is alkaline mesphilic lipase with strong tolerance for the surfactants, and can be applied to the industry of washing.

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