The invention discloses a low carbon heating type strawberry substrate culture device. A plurality of drainage ditches are arranged at the bottom of a culture tank and water draining holes are formed between the drainage ditches; a bacteria coated heating layer composed of a plurality of edible fungus bags is arranged at the bottom of a compound culture substrate of the culture tank; and a non-woven fabric is arranged between the bacteria coated heating layer and the compound culture substrate. The invention also discloses a method for using the device for culturing. The method comprises the following steps: putting the bag-removed edible fungus bags into the bottom of the culture tank and paving the bacteria coated heating layer; paving the non-woven fabric above the bacteria coated heating layer; spraying water onto the non-woven fabric and tightly gluing with the edible fungus bags; placing a prefabricated compound culture substrate above the non-woven fabric; and planting the strawberry into the compound culture substrate and culturing. Compared with the prior art, the low carbon heating type strawberry substrate culture device has the characteristics of low cost, simple mounting, convenience in operation, low carbon, environmental protection and high heating and heat-insulating property.