The invention discloses a test tube rack with a simple temperature control function, wherein the test tube rack comprises a square base body, the upper surface of the square base body is provided with a plurality of holes, the holes extend into the interior of the base body to form a cylindrical cavity, and the base body is filled with hot water or a cold storage agent. According to the present invention, during the use, the test tube rack is placed in the refrigerator and is frozen, such that the low temperature environment can be ensured for a certain time, and the test tube rack is especially suitable for the antibody dilution, active substance preparation and the like in molecule experiments; the test tube rack can further be used for the hot-water thermal insulation environment; and the test tube rack can directly achieve the thermal insulation environment or the low temperature environment so as to conveniently perform the experimental operation.