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发明公开
CN107488634A 青色荧光蛋白标记的肿瘤细胞株、构建方法及应用 无效 - 驳回

青色荧光蛋白标记的肿瘤细胞株、构建方法及应用
摘要：
本发明公开了一种青色荧光蛋白标记的肿瘤细胞株、构建方法及应用，涉及肿瘤免疫学和光学成像技术领域，本发明通过青色荧光蛋白质粒转染和G418药物筛选，获取青色荧光蛋白标记的肿瘤细胞株，从而实现青色荧光蛋白标记的肿瘤细胞株在肿瘤免疫光学研究中的应用，如实现通过显微光学成像对CTL杀伤肿瘤细胞的全过程进行观察研究，或在体内实现对肿瘤微环境的多色标记和显微动态成像，或在肿瘤治疗过程中实现对肿瘤微环境中的肿瘤细胞和多种免疫细胞以及血管之间的相关作用进行实时动态光学成像观察。
摘要（英）：

The invention discloses a cyan fluorescent protein marked tumor cell strain, and a construction method and the application thereof, and relates to the technical fields of tumor immunology and optical imaging. The cyan fluorescent protein marked tumor cell strain is obtained through cyan fluorescent protein plasmid transfection and G418 medicine screening, so that the application of the cyan fluorescent protein marked tumor cell strain to tumor immunity optical research is realized, for example, the whole process of killing tumor cells by CTL is observed and researched through microscopic optical imaging, or multicolor marking and microscopic dynamic imaging of the tumor microenvironment are realized in vivo, or real-time dynamic optical imaging observation on the dependent interaction among the tumor cells, various immune cells and blood vessels in the tumor microenvironment is realized in the tumor treatment process.

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