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发明公开
CN107488731A 水稻抗褐飞虱基因BPH9基因内特异SNP共显性分子标记引物及应用 有权

水稻抗褐飞虱基因BPH9基因内特异SNP共显性分子标记引物及应用
摘要：
本发明公开了一种水稻抗褐飞虱基因BPH9内特异SNP共显性分子标记引物，属于植物分子育种领域。本标记引物及其序列分别为：BPH9-IF:ACCATTGTTAGGCAGTTGTTCA；BPH9-ER:ATTCGACTCCCTTTCTTGTTATCT；BPH9-IR:CAGCCTCCTGAAGAGATCTTTCA；BPH9-EF:AATGTCGCACCCAGCAGC。本发明利用该引物对水稻DNA样本进行PCR扩增，可快速判断待测水稻BPH9的基因型，方法简便快捷、成本低廉，可广泛应用于分子标记辅助选择育种或者水稻种质资源BPH9基因型的鉴定。
摘要（英）：

The invention discloses a specific SNP (Single Nucleotide Polymorphism) co-dominant molecular marker primer in a rice brown planthopper resistance gene BPH9 and belongs to the field of plant molecular breeding. The marker primer and a sequence thereof are shown as follows: BPH9-IF: ACCATTGTTAGGCAGTTGTTCA; BPH9-ER: ATTCGACTCCCTTTCTTGTTATCT; BPH9-IR: CAGCCTCCTGAAGAGATCTTTCA; BPH9-EF: AATGTCGCACCCAGCAGC. The primer disclosed by the invention is used for carrying out PCR (Polymerase Chain Reaction) amplification on a rice DNA (Deoxyribonucleic Acid) sample and can be used for rapidly judging a gene type of rice BPH9 to be detected; the method is simple, convenient and rapid, is low in cost and can be widely applied to molecular marker assisted selective breeding or identification of BPH9 gene types of rice germplasm resources.

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