The invention discloses a method for improving the specific toxicity of nano-silver particles. The method is characterized by using silver ions to improve the specific toxicity of the nano-silver particles. According to the method disclosed by the invention, the specific toxicity of the nano-silver particles is effectively improved by using extra silver ions; in addition, the method has the advantages that the operation is simple, and a used reagent is easy to obtain and high in practicality; and besides, the method is of important significance for in-depth study of a toxicity mechanism of nano-silver and expansion of the application of the nano-silver in the fields of sterilization and bacteria resistance.