The invention discloses silkworm breeding feed and a preparation method thereof. The feed comprises, by weight, 30-50 parts of mulberry leaves, 20-25 parts of defatted soybean flour, 5-10 parts of lettuce, 10-15 parts of asparagus lettuce, 10-15 parts of Siberian elm leaves, 10-15 parts of stipules, 5-6 parts of preservative, 80-100 parts of mulberry twigs, 30-45 parts of cauliflowers, 5-10 parts of jumble beads, 5-10 parts of jobstears seeds, 3-5 parts of wild mint herbs, 3-5 parts of grape skins and 10-15 parts of edible glue. According to the preparation method, mulberry twigs are separated and then squeezed to obtain juice, the mulberry leaves and the like serve as carriers, the juice and other components are mixed, and mixture is loaded on the carriers to obtain the silkworm breeding feed.