The invention discloses a fungus pileus and stipe separating device with a lifting device. The fungus pileus and stipe separating device with the lifting device includes a metal conveying belt and a material lifting mechanism, and the metal conveying belt is arranged on a drive roller and a driven roller in a sleeving mode; a cutter is mounted at the inner side of the upper layer of the metal conveying belt; shifting-back mechanisms are mounted on the metal conveying belt; a plurality of through holes are formed in the metal conveying belt, and the front ends of the through holes are wider than the rear ends of the through holes; the drive roller and the driven roller are mounted on a shell; the metal conveying belt includes an upper metal conveying belt body and a lower metal conveying belt body, and the through holes in the upper metal conveying belt body are larger than the through holes in the lower metal conveying belt body. The fungus pileus and stipe separating device with the lifting device can rapidly and accurately complete automatic cutting, separating and collecting of fungus pilei and stipes, the cutting efficiency is high, the operation is stable, the speed of separating the pilei from the stipes is increased, and the labor cost is saved.