The invention relates to a bacillus EAb-2 and application thereof, and belongs to the technical field of microorganism. The strain has the preservation code of CGMCC (China General Microbiological Culture Collection Center) No.14102. Fermentation liquid prepared from the strain has high lethal activity on caenorhabditis elegans and meloidogyne incognita; the growth promotion effect is achieved on eupatorium adenophorum; the supplementation can be realized on the further study on the invasion and diffusion mechanism of the eupatorium adenophorum; the plant growth promotion potential is also realized.