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发明公开
CN107502681A 一种A组轮状病毒快速实时荧光RT-PCR检测试剂盒 无效 - 驳回

一种A组轮状病毒快速实时荧光RT-PCR检测试剂盒
摘要：
本发明公开了一种A组轮状病毒快速实时荧光RT-PCR检测试剂盒，包括扩增引物：正向引物：5’-gttgatgctcaagatggagt-3’和反向引物：5’-acttcattgtaatcatattgaatacc-3’，还包括特异性荧光探针：5'-X-cagcaacaactgcagcttcaaaagaagtgt-Y-3'。本发明所述的试剂盒能够满足针对A组轮状病毒检测中的时效性要求，通过对反应体系中引物、探针、反应条件的设计，并使用了耐热DNA快速聚合酶，结合一步法荧光RT-PCR技术，达到快速、实时、定量检测A组轮状病毒的目的，灵敏度可达到102PFU/ml。
摘要（英）：

The invention discloses a group-A rotavirus rapid real-time fluorescent RT-PCR (reverse transcription-polymerase chain reaction) detection kit which comprises an amplification primer, a forward primer 5'-gttgatgctcaagatggagt-3', a reverse primer 5'-acttcattgtaatcatattgaatacc-3' and a specific fluorescent probe 5'-X-cagcaacaactgcagcttcaaaagaagtgt-Y-3'. The kit can meet the requirement of timeliness for group-A rotavirus detection, group-A rotaviruses are rapidly and quantitatively detected in real time according to one-step fluorescent RT-PCR technology by the design of the primers, the probe and reaction conditions in a reaction system and heat-resisting DNA (deoxyribonucleic acid) rapid polymerase, and sensitivity can reach 10<2> PFU/ml.

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