The invention belongs to the technical field of release force regulators, discloses an application of vinyl silicone oil containing hydroxyl groups at terminal as a release force regulator to preparation of a release agent, the release agent and a release film and particularly provides an application to preparation of an addition type organosilicon release agent. According to the application of the vinyl silicone oil containing the hydroxyl groups at the terminal as the release force regulator to preparation of the release agent, the structural formula of the vinyl silicone oil containing the hydroxyl groups at the terminal is shown in the description, wherein n and m represent constitutional repeating units, n ranges from 1 to 10, and m ranges from 0 to 6. The vinyl silicone oil containing the hydroxyl groups as the regulator contains double functional groups, namely, the hydroxyl groups and vinyl groups, thereby having double functions when applied to the release agent, substrate bonding firmness is enhanced by the hydroxyl groups, surface tension of the material is improved by introduction of the hydroxyl groups, the vinyl groups participate in hydrosilylation, compatibility with the release agent is enhanced, the release film with remarkably improved release force can be obtained, and the higher the content of the vinyl silicone oil is, the higher the release force of the release film is.