The invention relates to a vertical rotation type anti-flooding protection sealed partition door locking mechanism, and belongs to the technical field of protection equipment. The vertical rotation type anti-flooding protection sealed partition door locking mechanism comprises a reducer and a plurality of locking heads; the reducer is provided with a shell, a worm gear is arranged in the shell, a hand wheel is in transmission connection with a worm through the worm gear, the worm is connected with a vertically-arranged lead screw, vertical connecting rods A are connected to the upper and lower ends of the lead screw, and a plurality of hinge pieces are arranged on the vertical connecting rods A; the two sides of each hinge piece are hinged to one end of a horizontal connecting rod through a triangular rotating arm and a through connection piece in sequence, the other end of each horizontal connecting rod is fixedly connected with a vertical connecting rod B, and a locking head is connected to each vertical connecting rod B; and the middle of each horizontal connecting rod is further vertically provided with a locking head through a vertical through connection piece. By means of the vertical rotation type anti-flooding protection sealed partition door locking mechanism, the problems that a metro large-space vertical rotation type anti-flooding protection sealed partition door is large in dead load, complex in locking operation and poor in sealing effect can be effectively solved.