The invention discloses a butting and locking device and a butting and locking method for releasing and recovering a deep submergence vehicle. The device comprises a base, a butting and locking device and a clamping and jacking device, wherein the butting and locking device comprises a box body, a locking plate and an elastic jacking device; and the clamping and jacking device comprises a clamping and jacking drive, a clamping and jacking connecting rod, a clamping and jacking arm and a clamping and jacking wheel. The method comprises the following steps: (1) a mushroom-shaped head of the deep submergence vehicle enters an inserting hole and meanwhile enables the locking plate to overcome the elastic jacking elastic jacking device to overturn, the mushroom-shaped head of the deep submergence vehicle enters a cavity, and the elastic jacking device enables the locking plate to automatically reset; and (2) the clamping and jacking drive is started, and the clamping and jacking wheel clamps and jacks the deep submergence vehicle. By utilizing the device and method disclosed by the invention, in the process of recovering the deep submergence vehicle, the clamping and jacking device can clamp the deep submergence vehicle; and in the processes of releasing and recovering the deep submergence vehicle, pull force, thrust force and holding force are supplied to the deep submergence vehicle, so that the deep submergence vehicle can not shake and has good stability.