The invention discloses a PDMS structure transfer method capable of high shape maintenance, which can be used for the preparation of a PDMS flexible multilayer micro-nano structure. The method comprises the following steps: preparing a silicon template 2 of a PDMS structure to be transferred; preparing a PDMS transfer layer 5; slowly carrying out trial dripping of an accurate volume of a PDMS solution along the center of the silicon template, and waiting until the PDMS solution disperses from the surface center in all directions and slowly flows into various structural cavities, wherein the PDMS solution is requested not to overflow into areas outside the surface of the silicon template; and placing the PDMS transfer layer 5 on the side of the silicon template 2 with the PDMS structure, uniformly applying a pressure, placing the PDMS transfer layer 5 and the silicon template 2 in a thermostat for solidification, taking out the PDMS transfer layer 5 and the silicon template 2 after the solidification, and stripping the silicon template 2 from the PDMS transfer layer 5, so that the PDMS structure in the structural cavities of the silicon template 2 is transferred to the PDMS transfer layer 5 in a shape-maintained manner. The method provided by the invention has the advantages that the technique process is relatively simple, a significant guarantee is provided for a multilayer micro-nano structure preparation technique, and a new solution for the application of a transfer technique in other micro-nano structure preparation techniques is further provided.