The invention discloses a method for establishing a source of heredofamilial premature ovarian failure and inducing differentiation of totipotential stem cells to original genital ridge cells. The method is characterized in that adult cells of patients with heredofamilial premature ovarian failure are applied and reprogrammed into induced totipotential stem cells in vitro, and then are induced and differentiated into precursor cells of the oocytes, namely the original genital ridge cells. The method is a method for obtaining original genital ridge cells from patients with heredofamilial premature ovarian failure, lays foundation for further obtaining manure oocytes, and is expected to be applied in the field of reproductive medicine and translational medicine.