The invention discloses a fluorescent detection pond seat for high performance liquid chromatography and flow injection analysis. A laser light path hole, a circulating pond hole and a fluorescent collecting light path hole are included in the detection pond seat primarily. The axis of the laser light path hole is orthogonal to the plane of axes of the circulating pond hole and the fluorescent collecting light path hole, and the included angle between the axes of the circulating pond hole and the fluorescent collecting light path hole is 20-70 degrees; and the detection signal to noise ratio is improved obviously by means of the orthogonal light path and the 20-70 degree fluorescent collecting angle. The inner walls of the circulating pond hole and the fluorescent collecting light path hole are blackened, so that the signal to noise ratio is further improved. The detection pond seat integrates all optical parts of the laser light path, the circulating pond and the fluorescent collecting light path, so that miniaturization and integration are achieved.