The invention discloses a manual breeding method of trochus niloticus. The method includes the steps of breeding area selection, benthic diatom cultivation, macro-algae planting, seeding, breeding management. According to the method, benthic diatom, gracilaria confervoides, gulfweed and other algae are planted and cultured for supplying sufficient food to the trochus niloticus in the breeding area, and meanwhile, by combining scientific and reasonable stocking density with an originally ecological breeding environment, the yield and growth speed of trochus niloticus are increased, and the method has the advantages of being low in breeding and production cost and capable of being popularized in a large scale.