The invention relates to the field of immunology, and particularly discloses a poultry ornithobacterium rhinotracheale vaccine. The vaccine is prepared by taking isolated strains of two main epidemicserotypes of ornithobacterium rhinotracheale as antigens, culturing with a blood bouillon culture-medium, then inactivating by formaldehyde, mixing the antigens of the two serotypes at a ratio of 1 to1, then adding poloxamer with volume being 1 to 5 percent of that of the liquid antigens and beta glucan with volume being 1 to 4 percent of that of the liquid antigens to prepare a water phase, finally emusifying according to a ratio of the water phase to an oil phase being 4 to 6. The vaccine provided by the invention can be used for preventing symptoms such as respiratory diseases caused by currently epidemic poultry ornithobacterium rhinotracheale, slow growth and egg-laying decrease.