The invention relates to pseudomonas aeruginosa and an application thereof. The pseudomonas aeruginosa is named as Pseudomonas aeruginosa YM4 according to bacterial strain classification, and has a preservation number of CCTCC NO:M 2017494. The bacterial strain can produce rhamnolipid in a high yield from hydrophilic carbon sources, hydrophilic carbon sources and composite carbon sources, and therhamnolipid has a substrate conversion rate reach 65-80%. In a rhamnolipid product of the YM4 bacterial strain, di-rhamnolipid and single rhamnolipid are main products, and the amount of di-rhamnolipid and single rhamnolipid takes 80-90% of the total amount of glycolipid. According to the carbon source metabolic characteristic of the bacterial strain, a foam-free seed medium formula suitable for industrial application is developed, and the contamination rate of a seed tank is greatly reduced. The bacterial strain is high in rhamnolipid yield, conversion rate and ratio of a diglycolipid component, and has high industrial application value.