The invention discloses a quantification analysis method of fish habitat space characteristics influenced by gate dam regulation and control; the method comprises the following steps: investigating fish resource present situations in a research area, and screening a target fish; aiming at the screened target fish, and using indoor stress experiments and history data to build a quantification response relation curve of the target fish with respect to key habitat factors; gathering research area basic hydrology and landform data so as to build a water-power water environment model, and combiningthe quantification response relation curve of the target fish with respect to key habitat factor so as to build a habitat model facing fish habitats; introducing a landscape ecology theory and methodso as to evaluate the space characteristics of the habitat simulation result facing the fish habitat, and quantitatively analyzing the influences on the fish habitat space characteristics by the gatedam operations. The method can guide multi-gate dam river ecology restorations and protections, thus providing important theory and practice meanings for basin water resource planning and configurations.