The invention provides a medicine capable of controlling feline distemper as well as a preparation method and applications of the medicine. The medicine capable of controlling feline distemper comprises a culture supernatant of human umbilical cord mesenchymal stem cells containing bioactive factors, recombinant cat omega-interferon and granulocyte colony-stimulating factors, wherein the molecularweight of the bioactive factors is 3-100 Kda. The medicine can effectively control diseases caused by feline distemper viruses and alleviate the symptoms including lassitude, vomiting, decrease of leukocytes, and the like which are caused by virus infection. The medicine provided by the invention can be transported for a long distance and stored for a long term, is convenient to use, belongs to aspecific medicine aiming at feline panleukopenia virus, and has a broad application prospect.