The invention discloses a hierarchical suction filtration device and aims to provide a hierarchical suction filtration device which is convenient to operate and capable of avoiding the problem that the testing result accuracy is affected as an experiment sample can be contaminated when a filtering membrane is replaced. The hierarchical suction filtration device comprises a vacuuming box body and aswitchable hierarchical filtering device, wherein a sealed inner cavity is formed in the vacuuming box body; the vacuuming box body comprises a lower box body of which the upper end is opened, and anupper sealing cover for sealing the opening in the upper end of the lower box body; a vacuuming interface is formed in the outer side surface of the lower box body; a vertical inserting hole which iscommunicated with the sealed inner cavity of the vacuuming box body is formed in the upper surface of the upper sealing cover.